Purification

Target

CPX_00403_00493  

Purification ID

A16646  

Expression

M9 minimal + semet (Oct 28, 2013)  

Experiment Date Start

Oct 28, 2013  

Experiment Date End

Oct 30, 2013  

Person

Natalia Maltseva  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, IMAC-II, SEC::SEC peak 1::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleaved  

SeMet Status

semet  

Covalent Modification

 

Final Buffer

20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  

Final Concentration (mg/mL)

18  

Final Volume (μL)

1400  

Shipped To Lab Id

ANL  

Shipped Date

Oct 30, 2013  

Inserted

Jul 02, 2014