Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP05616
Purification ID: N1
Expression: 2xYT (Feb 28, 2014)
Experiment Date Start: Sep 15, 2014
Experiment Date End
Person: Salvatore Nocadello
Lab: Northwestern University
Protocol: NU_default_purification
Status: success
Description
Affinity Tag Status: uncleaved
SeMet Status: native
Covalent Modification
Final Buffer: 50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole
Final Concentration (mg/mL): 13.2
Final Volume (μL): 500
Shipped To Lab Id
Shipped Date
Inserted: Jul 15, 2016

Experiment Date	Person	Lab	Protocol	Status	Method	Drop Precipitant Contents	Screen	pH	Plate Type
Sep 12, 2016	Salvatore Nocadello	Northwestern University	standard crystallization	n/a	sitting drop	Sodium Malonate		7	Protein: 7.0 mg/ml, 0.5M Sodium chloride, 0.05M Tris-HCl pH=7.2, 1/100 (v/v) Elastase, 2mM CB1. Crystallization condiction: Sodium Malonate 2M, pH 7
Sep 30, 2014	Salvatore Nocadello	Northwestern University	standard crystallization	crystal	sitting drop	Peg	QIAGEN/JCSG
Sep 02, 2016	Salvatore Nocadello	Northwestern University	WU_crystallization_protocol_1	small crystal	sitting drop	Sodium Malonate	Unspecified	7	Protein: 7.0 mg/ml, 0.5M Sodium chloride, 0.05M Tris-HCl pH=7.2, 1/100 (v/v) Elastase, 2mM CB1. Crystallization condiction: Sodium Malonate 2M, pH 7