Purification

Target

CPX_63254_63255  

Purification ID

A20596  

Expression

LB + 40 mM K2HPO4 (Jun 05, 2016)  

Experiment Date Start

Jun 10, 2016  

Experiment Date End

Jun 12, 2016  

Person

Robert Jedrzejczak  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, SEC::SEC peak 1::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleaved  

SeMet Status

native  

Covalent Modification

 

Final Buffer

50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  

Final Concentration (mg/mL)

19  

Final Volume (μL)

450  

Shipped To Lab Id

ANL  

Shipped Date

Jun 12, 2016  

Inserted

Jul 27, 2016