Purification

Target
CPX_06552_06553  
Purification ID
A20781  
Expression
M9 minimal + semet (Aug 04, 2015)  
Experiment Date Start
Aug 07, 2015  
Experiment Date End
Aug 10, 2015  
Person
Robert Jedrzejczak  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II, SEC::ÄKTAxpress using IMAC-I program::ÄKTAxpress IMAC-I program eluate (250 mM imidazole)  
Affinity Tag Status
cleaved  
SeMet Status
semet  
Final Buffer
50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  
Final Concentration (mg/mL)
1  
Final Volume (μL)
100000  
Shipped To Lab Id
ANL  
Shipped Date
Aug 10, 2015  
Inserted
Sep 08, 2016