Purification

Target
IDP02668  
Purification ID
S1  
Expression
2xYT (Feb 12, 2009)  
Experiment Date Start
Mar 30, 2009  
Experiment Date End
 
Person
Charitha Nakka  
Lab
University of Texas Southwestern Medical Center  
Protocol
UTSW_standard purification  
Status
success  
Description
We observed 2 peaks after Q-column. Pooled those fractions and run gel filtration independently. Observed 2 peaks for each gelfiltration column. Finally we ended up with 4 peaks. Pooled and concentrated all 4 peak fractions. Observed ceryals in B peak.  
Affinity Tag Status
cleaved  
SeMet Status
na  
Final Buffer
Gel filtration Buffer (1L) - 200mM NaCl + 50mM Hepes + 1mM DTT  
Final Concentration (mg/mL)
16.63  
Final Volume (μL)
900  
Inserted
Oct 15, 2009