Purification

Target

IDP90521  

Purification ID

S1  

Expression

2xYT (Oct 28, 2009)  

Experiment Date Start

Nov 17, 2009  

Experiment Date End

 

Person

Charitha Nakka  

Lab

University of Texas Southwestern Medical Center  

Protocol

UTSW_standard purification  

Status

success  

Description

Extended the dialysis step by adding the additonal TEV Protease to the dialysis bag for 3hrs.  

Affinity Tag Status

cleaved  

SeMet Status

native  

Covalent Modification

 

Final Buffer

dialysis buffer II - 500mM NaCl +50mM HEPES+1mM DTT  

Final Concentration (mg/mL)

71.18  

Final Volume (μL)

1500  

Shipped To Lab Id

 

Shipped Date

 

Inserted

Dec 31, 2009