Purification

Target
CPX_63257_63258  
Purification ID
A21303  
Expression
LB + 40 mM K2HPO4 (Nov 01, 2016)  
Experiment Date Start
Mar 28, 2017  
Experiment Date End
Mar 30, 2017  
Person
Grazyna Joachimiak  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II, SEC::SEC peak 1::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
na  
SeMet Status
native  
Final Buffer
50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  
Final Concentration (mg/mL)
24  
Final Volume (μL)
270  
Shipped To Lab Id
ANL  
Shipped Date
Mar 30, 2017  
Inserted
May 17, 2017