Purification

Target
IDP05773  
Purification ID
W1  
Expression
LB (Feb 02, 2009)  
Experiment Date Start
Feb 03, 2009  
Experiment Date End
Feb 04, 2009  
Person
Chris Nelson  
Lab
Washington University  
Protocol
WU_native_inclusion_body_expression_2  
Status
success  
Description
Arginine refolding, followed by gel filtration on Superdex 200 16/60 HR column.  
Affinity Tag Status
uncleaved  
SeMet Status
native  
Final Buffer
20 mM Hepes pH=7.4, 20 mM NaCl, 0.01% NaAzide  
Final Concentration (mg/mL)
15  
Final Volume (μL)
100  
Shipped To Lab Id
WU  
Shipped Date
Feb 04, 2009  
Inserted
Aug 12, 2010