Purification

Target
IDP00257  
Purification ID
W1  
Expression
Athena_SeMET (Oct 29, 2008)  
Experiment Date Start
Oct 31, 2008  
Experiment Date End
Oct 31, 2008  
Person
Chris Nelson  
Lab
Washington University  
Protocol
WU_native_inclusion_body_expression_2  
Status
success  
Description
Arginine refolding, followed by gel filtration on Superdex 200 16/60 HR column.  
Affinity Tag Status
uncleaved  
SeMet Status
semet  
Final Buffer
20 mM Hepes pH=7.4, 20 mM NaCl, 0.01% NaAzide  
Final Concentration (mg/mL)
15  
Final Volume (μL)
150  
Shipped To Lab Id
WU  
Shipped Date
Oct 31, 2008  
Inserted
Nov 06, 2008