Purification

Target
IDP00012  
Purification ID
A3874  
Expression
M9 minimal (Sep 03, 2008)  
Experiment Date Start
Sep 10, 2008  
Experiment Date End
Sep 12, 2008  
Person
Minyi Gu  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, SEC, IMAC-II::SEC::SEC peak 1  
Affinity Tag Status
cleaved  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
29  
Final Volume (μL)
2800  
Shipped To Lab Id
ANL  
Shipped Date
Sep 12, 2008  
Inserted
Jul 29, 2011