Purification

Target
IDP90797  
Purification ID
A2027  
Expression
M9 minimal (Nov 10, 2009)  
Experiment Date Start
Nov 13, 2009  
Experiment Date End
Nov 17, 2009  
Person
Magdalena Makowska-Grzyska  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
cleaved  
SeMet Status
native  
Final Buffer
50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  
Final Concentration (mg/mL)
151  
Final Volume (μL)
750  
Shipped To Lab Id
ANL  
Shipped Date
Nov 17, 2009  
Inserted
Jul 29, 2011