Purification

Target
IDP91761  
Purification ID
A7907  
Expression
LB (Jan 10, 2012)  
Experiment Date Start
Jan 12, 2012  
Experiment Date End
Jan 14, 2012  
Person
Boguslaw Nocek  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
PIG-I, IMAC-II, SEC::SEC peak 1::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
cleaved  
SeMet Status
native  
Final Buffer
50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  
Final Concentration (mg/mL)
59  
Final Volume (μL)
1500  
Shipped To Lab Id
ANL  
Shipped Date
Jan 14, 2012  
Inserted
Jan 24, 2012