The antibiotic vancomycin exerts its action by binding to an intermediate component of the bacterial cell wall synthesis pathway and inhibits the maturation of the cell wall. Vancomycin resistance type VanG is mediated by enzymes that modify peptidoglycan cell wall precursors such that they contain serine substituted for alanine; this single point mutation results in drastic decrease in the binding of vancomycin. One of the genes in the VanG operon is VanUg, which is a predicted transcription regulator of the expression of the VanG-type vancomycin resistance enzymes. This crystal structure shows VanUg adopts a helix-turn-helix (HTH) fold, typical of many bacterial transcription factors. This structure may assist with the understanding of the biology of VanG-type vancomycin resistance, and therefore may assist with the development of novel therapeutics to alleviate this type of antibiotic resistance.