Structure of IDP91868
Crystal structure of aminoglycoside antibiotic 6'-N-acetyltransferase AAC(6')-Ig from Acinetobacter haemolyticus, apo
Edit deposit information
- CSGID target
- IDP91868
- PDB Id
- 4F0Y (NCBI MMDB)
- Authors
- P.J.Stogios,E.Evdokimova,A.Dong,G.Minasov,V.Yim,P.Courvalin,A.Savchenko,W.F.Anderson,Center For Structural Genomics Of Infectious Diseases (Csgid)
- Responsible person
- Peter Stogios
- Responsible lab
- University of Calgary
- Deposition Date
- May 05, 2012
- Release Date
- May 16, 2012
Annotation
- Description
- Resistance to aminoglycoside antibiotics is mediated by chemical modifications including phosphorylation, nucleotidylation and acetylation. The largest family of aminoglycoside-modifying enzymes are the amino glycoside 6'-N-acetyltransferase (AAC) enzymes. There are at least 60 of these enzymes but only structural knowledge of five at the time of this structure determination. Here we present the apo structure of AAC(6')-Ig, an enzyme from the emerging pathogen Acinetobacter haemolyticus.
- Functional assignment
- antibiotic resistance
Ligands
| Ligand code | Name | Ligand type |
|---|---|---|
| CL | chloride | crystallization |
| MG | magnesium | crystallization |
| 175 | 3,5-dihydro-5-methylidene-4h-imidazol-4-on |
Structure information
Unit cell parameters
- Space Group
- P 1
- Unit Cell
-
a=37.96Å, b=44.02Å, c=46.58Å
α=83.19, β=87.44, γ=72.65 - Solvent content
- Matthews coefficient
- Resolution range
- 24.07-2.56Å (2.72-2.56Å)
- Rall(%)
- 18.4
- Rwork(%)
- 18.1 (25.9)
- Rfree(%)
- 24.0 (37.8)
- Num. observed reflections
- 9328 (2497)
- Num. Rfree reflections
- 473 (130)
- Completeness(%)
- 85.3 (81.0)
- Num Atoms
- 2316
- Num Waters
- 84
- Num Hetatoms
- 101
- Model mean isotropic B factor
- 39.220Å2
- RMSD bond length
- 0.004Å
- RMSD bond angle
- 0.655°
- RMSD dihedral angle
- 12.953°
- Filename uploaded
- 4F0Y.pdb (uploaded on May 16, 2012 9:44 AM)
- Inserted
- May 05, 2012