Annotation

Description

Ornithine carbamoyltransferase (OTC) catalyzes the reversible transfer of the carbamoyl group from carbamoyl phosphate (CP) to the Nε atom of L-ornithine (ORN) to produce L-citrulline. There are two types of the enzyme – anabolic (aOTC) and catabolic (cOTC). Anabolic OTCs participate in the urea cycle and L-arginine biosynthesis. Catabolic OTCs are part of the catabolic arginine deiminase pathway found in a number of microorganisms. The reported structure is for the anabolic enzyme from pathogen Vibrio vulnificus. The structure has three monomers in the asymmetric unit which form the physiologically active trimer. All three enzyme subunits are unliganded. This structure provides insights into native state of the enzyme featuring open interdomain cleft, disordered SMG loop and catalytic loop B2–H3 located out of the active site. 

Functional assignment

transferase 

Structure information

Unit cell parameters

Space Group

P 21 21 21  

Unit Cell

a=77.81Å, b=82.65Å, c=150.91Å
α=90.00, β=90.00, γ=90.00 

Solvent content

 

Matthews coefficient

 

Refinement

Data for the highest resolution shell is in parentheses.

Resolution range

35.50-1.86Å (1.91-1.86Å)  

R_all(%)

16.1 

R_work(%)

16.0 (25.9) 

R_free(%)

19.5 (30.4) 

Num. observed reflections

81046 (5944) 

Num. R_free reflections

4052 (300) 

Completeness(%)

98.3 (98.6) 

Model parameters

Num Atoms

7535  

Num Waters

487  

Num Hetatoms

501  

Model mean isotropic B factor

39.730Ų  

RMSD bond length

0.019Å  

RMSD bond angle

1.738°  

Filename uploaded

hkl_refine_62_tls_ters.pdb (uploaded on May 24, 2013 2:40 PM)  

Inserted

May 24, 2013