Peptide deformylase is an intracellular target now well-recognized for the design of new antibiotics. The bacterial susceptibility to such a cytoplasmic target primarily depends on the capacity of the compound to reach and accumulate in the cytosol. Human peptide deformylase (hPDF), located in the mitochondria, has recently become a promising target for anti-cancer therapy. Our crystal structures of polypeptide deformylase (PDF) of Vibrio cholerae shows the similarity with that of other bacterials, like E. coli., staphylococcus aureus and thermotoga.