Annotation

Description

Phosphoribosylamine-glycine ligase (also known as glycinamide ribonucleotide synthetase (GAR-syn) and PurD protein) catalyzes the second step of the de novo purine biosynthetic pathway; the conversion of phosphoribosylamine (PRA), glycine, and ATP to glycinamide ribonucleotide (GAR), ADP, and phosphate. In the reaction, the glycine is ligated to the PRA amino group in an ATP-dependent manner and provides atoms C(4), C(5) and N(7) of the purine base. The enzyme is a member of the ATP-grasp superfamily. 

Functional assignment

 

Structure information

Unit cell parameters

Space Group

P 21 21 21  

Unit Cell

a=68.64Å, b=69.15Å, c=95.17Å
α=90.00, β=90.00, γ=90.00 

Solvent content

49.73  

Matthews coefficient

2.45  

Refinement

Data for the highest resolution shell is in parentheses.

Resolution range

30.80-1.47Å (1.51-1.47Å)  

R_all(%)

13.7 

R_work(%)

13.5 (22.1) 

R_free(%)

17.4 (27.7) 

Num. observed reflections

77708 (5547) 

Num. R_free reflections

3885 (287) 

Completeness(%)

99.7 (97.1) 

Model parameters

Num Atoms

4022  

Num Waters

512  

Num Hetatoms

0  

Model mean isotropic B factor

13.690Ų  

RMSD bond length

0.018Å  

RMSD bond angle

1.740°  

Filename uploaded

idp04490.pdb (uploaded on Apr 14, 2010 12:46 PM)  

Inserted

Apr 14, 2010