Pantoate-beta-alanine ligase (pantothenate synthase) participates in pantothenate biosynthesis pathway by catalyzing the formation of (R)-pantothenate from D-pantoate and beta-alanine. (R)-pantothenate is also called vitamin B5 or D-pantothenic acid. Micro-organisms and plants must synthesize pantothenate, while animals obtain this essential nutrient from their diet. Enzymes from pantothenate biosynthesis pathway offer targets for developing drugs against microbial pathogens. The crystal structure of Pantoate-beta-alanine ligase complexed with AMP from Francisella tularensis subsp. tularensis SCHU S4 was solved to 2A resolution.