Pyridoxal phosphate biosynthetic protein PdxJ is involved in the de novo synthesis of pyridoxal 5'-phosphate (Vitamin B6). Vitamin B6 is necessary for over 100 enzymatic reactions. It is also implicated in numerous human body functions ranging from modulation of hormone function to its recent discovery as a potent antioxidant. Its de novo biosynthesis occurs only in bacteria, fungi and plants. PdxJ protein catalyzes the condensation of 1-deoxy-D-xylulose-5-phosphate (DXP) and 1-amino-3-oxo-4-(phosphohydroxy)propan-2-one to form pyridoxal 5'-phosphate. PdxJ is a potential target for the development of new antibiotics due to its absence in mammals.