Annotation

Description

Poxvirus have evolved an array of mechanisms to circumvent the effects of type I interferons (IFNs), a group of cytokines known for their ability to confer resistance to viral infection. For example, the virulent rodent pathogen Ectromelia virus, that causes mousepox, encodes a secreted decoy receptor named EVM166 that is able to bind and inhibit type-I INFs. This entry contains a crystal structure of EVM166 in complex with murine IFNα5 and reveals a striking similarity between EVM166 and the ectodomain of the human receptor for IL-1β. The regions buried by EVM166 on IFNα5 are thought to be crucial for its’ interactions with the host-encoded INF-α receptor (a cell-surface heterodimer consisting of the two chains IFNAR1 and IFNAR2). Many species within the orthopoxvirus genus encode decoy receptors for type-I INFs that have sequence similarity to EVM166. These include Vaccinia, Cowpox, and Variola, among others. Sub-species that lack or encode truncated versions of EVM166 display a significantly attenuated phenotype in mice demonstrating the importance of this decoy receptor as a virulence factor. The information contained in this entry may prove useful in the future development of antiviral therapeutics targeted against poxviruses. It may also prove useful in the design and creation of novel anti-inflammatory therapeutics.  

Functional assignment

virulence factor 

Structure information

Unit cell parameters

Space Group

P 21 21 21  

Unit Cell

a=48.84Å, b=75.90Å, c=182.73Å
α=90.00, β=90.00, γ=90.00 

Solvent content

 

Matthews coefficient

 

Refinement

Data for the highest resolution shell is in parentheses.

Resolution range

43.07-2.10Å (2.18-2.10Å)  

R_all(%)

20.4 

R_work(%)

20.3 (0.0) 

R_free(%)

23.5 (0.0) 

Num. observed reflections

71824 (7650) 

Num. R_free reflections

3519 (382) 

Completeness(%)

95.9 (92.5) 

Model parameters

Num Atoms

3974  

Num Waters

1  

Num Hetatoms

417  

Model mean isotropic B factor

35.890Ų  

RMSD bond length

0.000Å  

RMSD bond angle

0.000°  

Filename uploaded

pdb_extract_coord_15251.cif (uploaded on Sep 13, 2010 4:21 PM)  

Inserted

Sep 13, 2010