Azoreductase (AzoR) is an FMN-dependent NADH-azoreductase isolated from Bacillus authracis str. Sterne as a protein responsible for the degradation of azo compounds.This enzyme catalyzes the reductive cleavage of azo groups by a ping-pong mechanism. The structure suggests that AzoR acts in a homodimeric state. The structure revealed that AzoR has a flavodoxin-like structure, without the explicit overall amino acid sequence homology. In the FMN-binding site the Sulphate ion was identified.
FMN-dependent; requires NADH; catalyzes the cleavage of azo bond in aromatic azo compounds