Phosphoribosylaminoimidazole synthetase is the fifth enzyme in the de novo synthesis of purine nucleotides. It catalyzes the reaction to form 5-aminoimidizole ribonucleotide (AIR) from formylglycinamidine-ribonucleotide FGAM. This reaction closes the ring and produces a 5-membered imidazole ring of the purine nucleus (AIR). AIR synthetase catalyzes the transfer of the oxygen of the formyl group to phosphate. It is a sequential mechanism in which ATP binds first to the enzyme and ADP is released last. This enzyme hydrolyzes ATP to activate the oxygen of the amide in order to carry out a nucleophilic attack by nitrogen. Crystal Structure of Phosphoribosylaminoimidazole Synthetase from Francisella tularensis was solved at 1.8A in the presence of pyrophosphate. Overall structure represents dimer, each monomer consisting of three domains, the N-terminal alpha-beta domain, the small alpha-beta domain, and the small alpha domain. The N-terminal large alpha-beta domain interacts with the N-terminal domain of the other monomer using 4-stranded beta-sheet. The active site is proposed to be the groove between the two beta-sheets from the two monomers.