Center for Structural Genomics of Infectious Diseases - Deposits

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Structure of IDP04610

Crystal Structure of Methyionyl-tRNA Formyltransferase from Bacillus anthracis

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CSGID target: IDP04610
PDB Id: 3RFO (NCBI MMDB)
Authors: Y.Kim,M.Makowska-Grzyska,K.Kwon,W.F.Anderson,A.Joachimiak,Csgid,Center For Structural Genomics Of Infectious Diseases (Csgid)
Responsible person: Youngchang Kim
Responsible lab: Argonne National Laboratory
Deposition Date: Apr 06, 2011
Release Date: Apr 20, 2011

Annotation

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Description: Methionyl-tRNA formyltransferase modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet) at the beginning of translation initiation essential for normal bacterial growth. The structure contains the bigger N-terminal Rossman-fold domain and the C-terminal small beta-barrel domain resembling an OB-fold, positively charged in one side, which presumably interacts with a corresponding tRNA.
Functional assignment: transferase

Ligands

Ligand code	Name	Ligand type
SO4	sulfate	crystallization
PG5		crystallization
MSE		modified residue
SO4	sulfate	crystallization
GOL	glycerol	crystallization

Structure information

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Unit cell parameters

Space Group: P 1 21 1
Unit Cell: a=62.86Å, b=191.50Å, c=89.95Å
α=90.00, β=89.95, γ=90.00
Solvent content
Matthews coefficient
Resolution range: 36.59-2.38Å (2.42-2.40Å)
R_all(%): 18.3
R_work(%): 18.1 (28.3)
R_free(%): 21.0 (31.5)
Num. observed reflections: 87076 (3309)
Num. R_free reflections: 4379 (154)
Completeness(%): 97.5 (75.0)
Num Atoms: 9855
Num Waters: 504
Num Hetatoms: 558
Model mean isotropic B factor: 58.150Å²
RMSD bond length: 0.004Å
RMSD bond angle: 0.900°
RMSD dihedral angle: 14.03°
Filename uploaded: dep.pdb (uploaded on Jan 11, 2013 4:34 PM)
Inserted: Apr 06, 2011