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Structure of IDP04610

Crystal Structure of Methyionyl-tRNA Formyltransferase from Bacillus anthracis

Edit deposit information
CSGID target
IDP04610 
PDB Id
3RFO (NCBI MMDB
Authors
Y.Kim,M.Makowska-Grzyska,K.Kwon,W.F.Anderson,A.Joachimiak,Csgid,Center For Structural Genomics Of Infectious Diseases (Csgid) 
Responsible person
Youngchang Kim 
Responsible lab
Argonne National Laboratory 
Deposition Date
Apr 06, 2011 
Release Date
Apr 20, 2011 

Annotation

Description
Methionyl-tRNA formyltransferase modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet) at the beginning of translation initiation essential for normal bacterial growth. The structure contains the bigger N-terminal Rossman-fold domain and the C-terminal small beta-barrel domain resembling an OB-fold, positively charged in one side, which presumably interacts with a corresponding tRNA.  
Functional assignment
transferase 

Ligands

Ligand code Name Ligand type
SO4 sulfate molecule crystallization
PG5 crystallization
MSE modified residue
SO4 sulfate molecule crystallization
GOL glycerol crystallization

Structure information

Unit cell parameters

Space Group
P 1 21 1  
Unit Cell

a=62.86Å, b=191.50Å, c=89.95Å
α=90.00, β=89.95, γ=90.00 
Solvent content
 
Matthews coefficient
 

Refinement

Data for the highest resolution shell is in parentheses.
Resolution range
36.59-2.38Å (2.42-2.40Å)  
Rall(%)
18.3 
Rwork(%)
18.1 (28.3) 
Rfree(%)
21.0 (31.5) 
Num. observed reflections
87076 (3309) 
Num. Rfree reflections
4379 (154) 
Completeness(%)
97.5 (75.0) 

Model parameters

Num Atoms
9855  
Num Waters
504  
Num Hetatoms
558  
Model mean isotropic B factor
58.150Å2  
RMSD bond length
0.004Å  
RMSD bond angle
0.900°  
RMSD dihedral angle
14.03°
 
Filename uploaded
dep.pdb (uploaded on Jan 11, 2013 4:34 PM)  
Inserted
Apr 06, 2011