Structure of CPX_52010_52016

Crystal Structure of SARS-CoV-2 Nsp16/10 Heterodimer in Complex with (m7GpppA2m)pUpUpApApA (Cap-1), S-Adenosyl-L-homocysteine (SAH) and Manganese (Mn).

Edit deposit information
CSGID target
CPX_52010_52016 
PDB Id
7L6R (NCBI MMDB
Authors
Minasov, G., Shuvalova, L., Rosas-Lemus, M., Kiryukhina, O., Brunzelle, J.S., Satchell, K.J.F., Center for Structural Genomics of Infectious Diseases (CSGID). 
Responsible person
George Minasov 
Responsible lab
Northwestern University 
Deposition Date
Dec 23, 2020 
Release Date
Jan 06, 2021 

Annotation

Ligands

Ligand code Name Ligand type
M7G 7n-methyl-8-hydroguanosine-5'-diphosphate biological
A2M 2'-o-methyl-adenosine-5'-monophosphate biological
MN biological
CL chloride ion crystallization
SO4 crystallization
SAH modified residue
GLC crystallization
ZN biological
BDF beta-d-fructopyranose crystallization
U unknown biological
A unknown biological

Structure information

Unit cell parameters

Space Group
P 31 2 1  
Unit Cell

a=168.76Å, b=168.76Å, c=52.27Å
α=90.00, β=90.00, γ=120.00 
Solvent content
 
Matthews coefficient
 

Refinement

Data for the highest resolution shell is in parentheses.
Resolution range
29.95-1.98Å (2.03-1.98Å)  
Rall(%)
15.1 
Rwork(%)
15.0 (27.8) 
Rfree(%)
16.6 (25.8) 
Num. observed reflections
59437 (4345) 
Num. Rfree reflections
3090 (211) 
Completeness(%)
99.9 (99.2) 

Model parameters

Num Atoms
3368  
Num Waters
341  
Num Hetatoms
505  
Model mean isotropic B factor
43.990Å2  
RMSD bond length
0.005Å  
RMSD bond angle
1.272°  
Filename uploaded
7l6r.pdb (uploaded on Jan 11, 2021 2:40 PM)  
Inserted
Jan 11, 2021