Structure of IDP97791

Powassan Virus Envelope Protein DIII In Complex With Neutralizing Fab

Edit deposit information
CSGID target
IDP97791 
PDB Id
7KYL (NCBI MMDB
Authors
Errico, J.M., Nelson, C.A., Fremont, D.H., Center for Structural Genomics of Infectious Diseases (CSGID) 
Responsible person
Chris Nelson 
Responsible lab
Washington University 
Deposition Date
Dec 08, 2021 
Release Date
 

Annotation

Description
Powassan virus (POWV) is an emerging tick-transmitted flavivirus that causes severe or fatal neuroinvasive disease in humans. The antibody POWV-80 binds domain III (DIII) of the POWV envelope protein with high affinity and potentially neutralizes several tick-borne flaviviruses in addition to POWV, including Langat and tick-borne encephalitis virus. This structure defines the footprint of POWV-80 on the POWV DIII domain.  
Functional assignment
VIRULENCE 

Ligands

Ligand code Name Ligand type
PCA modified residue
NA biological
CL biological

Structure information

Unit cell parameters

Space Group
P 1 21 1  
Unit Cell

a=79.15Å, b=62.56Å, c=141.55Å
α=90.00, β=97.97, γ=90.00 
Solvent content
 
Matthews coefficient
 

Refinement

Data for the highest resolution shell is in parentheses.
Resolution range
46.67-2.00Å (2.09-2.00Å)  
Rall(%)
19.1 
Rwork(%)
19.0 (24.5) 
Rfree(%)
22.4 (27.3) 
Num. observed reflections
94056 (11417) 
Num. Rfree reflections
1091 (137) 
Completeness(%)
99.9 (100.0) 

Model parameters

Num Atoms
8186  
Num Waters
1088  
Num Hetatoms
1106  
Model mean isotropic B factor
33.890Å2  
RMSD bond length
0.000Å  
RMSD bond angle
0.000°  
Filename uploaded
POWV80-POWVDIII.pdb (uploaded on Jun 14, 2021 4:29 PM)  
Inserted
Jun 14, 2021