Center for Structural Genomics of Infectious Diseases

Expression

Target: IDP00487
Expression ID: A1135
Protein Clone: pMCSG19c (Jun 06, 2008)
Experiment Date Start: Oct 14, 2008
Experiment Date End: Oct 16, 2008
Person: Min Zhou
Lab: Argonne National Laboratory
Protocol: standard expression_ANL
Status: failed
Expression Organism: E. coli
Expression Strain: BL21(DE3)magic
Media Type: M9 minimal + semet
Media Volume: 2000 mL
Growth Temperature: 37 °C
Induction Temperature: 16 °C
Induction Reagent: 1 mM IPTG
Experiment Type: production
Expression Level: 100.0 %
Solubility Level: 0.0 %
Inserted: Jul 29, 2011

Related Purifications

Experiment Start Date	Experiment End Date	Person	Lab	Protocol	Status	Affinity Tag Status	SeMet Status	Final Buffer	Shipped To Lab	Shipped Date
Oct 21, 2008	Oct 23, 2008	Min Zhou	Argonne National Laboratory	standard purification_ANL	success	na	semet	50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	ANL	2008-10-23
Oct 21, 2008	Oct 23, 2008	Min Zhou	Argonne National Laboratory	standard purification_ANL	success	na	semet	50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	ANL	2008-10-23
Oct 21, 2008	Oct 23, 2008	Min Zhou	Argonne National Laboratory	standard purification_ANL	success	na	semet	50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	ANL	2008-10-23
Oct 21, 2008	Oct 23, 2008	Min Zhou	Argonne National Laboratory	standard purification_ANL	success	na	semet	50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	ANL	2008-10-23
Oct 21, 2008	Oct 23, 2008	Min Zhou	Argonne National Laboratory	standard purification_ANL	success	na	semet	50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	ANL	2008-10-23
Oct 21, 2008	Oct 23, 2008	Min Zhou	Argonne National Laboratory	standard purification_ANL	success	na	semet	50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	ANL	2008-10-23
Oct 21, 2008	Oct 23, 2008	Min Zhou	Argonne National Laboratory	standard purification_ANL	success	na	semet	50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	ANL	2008-10-23