Expression

Target
IDP06401  
Expression ID
J2  
Protein Clone
pMCSG7-Halo (Nov 20, 2013)  
Experiment Date Start
Oct 17, 2013  
Experiment Date End
Oct 17, 2013  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins  
Status
success  
Expression Organism
E. coli  
Expression Strain
BL21(DE3)-Magic  
Media Type
2xYT  
Media Volume
0.15 mL  
Growth Temperature
37 °C  
Induction Temperature
25 °C  
Induction Reagent
1mM IPTG  
Experiment Type
assay  
Expression Level
30.0 %  
Solubility Level
100.0 %  
Inserted
Nov 22, 2013