Expression

Target
IDP00269  
Expression ID
W310  
Protein Clone
pET21A(+) (Jan 20, 2011)  
Experiment Date Start
Apr 04, 2012  
Experiment Date End
 
Person
Chris Nelson  
Lab
Washington University  
Protocol
WU_native_inclusion_body_expression_2  
Status
success  
Expression Organism
E. coli  
Expression Strain
BL21-CodonPlus(DE3)-RIL  
Media Type
Athena_SeMET  
Media Volume
1000 mL  
Growth Temperature
20 °C  
Induction Temperature
20 °C  
Induction Reagent
0.2 mM IPTG  
Experiment Type
production  
Inserted
Feb 28, 2014  

Related Purifications

Experiment
Start
Date		 Experiment
End
Date		 Person Lab Protocol Status Affinity
Tag
Status		 SeMet
Status		 Covalent
Modification		 Final
Buffer		 Shipped
To
Lab		 Shipped
Date