Expression

Target
IDP06443  
Expression ID
J1  
Protein Clone
pMCSG53 (Jan 13, 2014)  
Experiment Date Start
Jan 23, 2014  
Experiment Date End
Jan 23, 2014  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins  
Status
success  
Expression Organism
E. coli  
Expression Strain
BL21(DE3)-Magic  
Media Type
2xYT  
Media Volume
0.15 mL  
Growth Temperature
37 °C  
Induction Temperature
25 °C  
Induction Reagent
1mM IPTG  
Experiment Type
assay  
Expression Level
20.0 %  
Solubility Level
100.0 %  
Inserted
Mar 14, 2014