Expression

Target
IDP00213  
Expression ID
A9095  
Protein Clone
pMCSG73 (Jan 06, 2014)  
Experiment Date Start
Oct 29, 2014  
Experiment Date End
Oct 31, 2014  
Person
Gekleng Chhor  
Lab
Argonne National Laboratory  
Protocol
standard expression_ANL  
Status
success  
Expression Organism
E. coli  
Expression Strain
BL21-Gold(DE3)  
Media Type
M9 minimal + semet  
Media Volume
3000 mL  
Growth Temperature
37 °C  
Induction Temperature
18 °C  
Induction Reagent
0.5 mM IPTG  
Experiment Type
production  
Expression Level
60.0 %  
Solubility Level
60.0 %  
Inserted
Jan 29, 2015  

Related Purifications

Experiment
Start
Date		 Experiment
End
Date		 Person Lab Protocol Status Affinity
Tag
Status		 SeMet
Status		 Covalent
Modification		 Final
Buffer		 Shipped
To
Lab		 Shipped
Date