Expression

Target
IDP01934  
Expression ID
J9  
Protein Clone
pMCSG53 (Nov 02, 2015)  
Experiment Date Start
Sep 15, 2015  
Experiment Date End
Sep 15, 2015  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins  
Status
success  
Expression Organism
E. coli  
Expression Strain
BL21(DE3)-Magic  
Media Type
2xYT  
Media Volume
0.15 mL  
Growth Temperature
37 °C  
Induction Temperature
25 °C  
Induction Reagent
1mM IPTG  
Experiment Type
assay  
Expression Level
25.0 %  
Solubility Level
80.0 %  
Inserted
Oct 30, 2015