Expression

Target
IDP97533  
Expression ID
A11794  
Protein Clone
pMCSG53 (Mar 07, 2019)  
Experiment Date Start
Nov 04, 2019  
Experiment Date End
Nov 06, 2019  
Person
Christine Tesar  
Lab
Argonne National Laboratory  
Protocol
standard expression_ANL  
Status
success  
Expression Organism
E. coli  
Expression Strain
BL21-Gold(DE3)  
Media Type
M9 minimal + semet  
Media Volume
4000 mL  
Growth Temperature
37 °C  
Induction Temperature
18 °C  
Induction Reagent
0.5 mM IPTG  
Experiment Type
production  
Expression Level
40.0 %  
Solubility Level
40.0 %  
Inserted
Nov 08, 2019  

Related Purifications

Experiment
Start
Date		 Experiment
End
Date		 Person Lab Protocol Status Affinity
Tag
Status		 SeMet
Status		 Covalent
Modification		 Final
Buffer		 Shipped
To
Lab		 Shipped
Date