Expression

Target
IDP51000  
Expression ID
A11885  
Protein Clone
pMCSG53 (Mar 09, 2020)  
Experiment Date Start
Mar 20, 2020  
Experiment Date End
Mar 22, 2020  
Person
Robert Jedrzejczak  
Lab
Argonne National Laboratory  
Protocol
standard expression_ANL  
Status
success  
Expression Organism
E. coli  
Expression Strain
BL21-Gold(DE3)  
Media Type
M9 minimal + semet  
Media Volume
1000 mL  
Growth Temperature
37 °C  
Induction Temperature
18 °C  
Induction Reagent
0.5 mM IPTG  
Experiment Type
production  
Expression Level
80.0 %  
Solubility Level
80.0 %  
Inserted
Jun 03, 2020