Expression

Target
IDP98885  
Expression ID
A12144  
Protein Clone
pMCSG53 (Nov 22, 2021)  
Experiment Date Start
Sep 01, 2021  
Experiment Date End
Sep 03, 2021  
Person
Christine Tesar  
Lab
Argonne National Laboratory  
Protocol
standard expression_ANL  
Status
success  
Expression Organism
E. coli  
Expression Strain
BL21 (DE3) pGrow7-K  
Media Type
LB + 40 mM K2HPO4  
Media Volume
2000 mL  
Growth Temperature
37 °C  
Induction Temperature
18 °C  
Induction Reagent
0.5 mM IPTG  
Experiment Type
production  
Expression Level
80.0 %  
Solubility Level
80.0 %  
Inserted
Jan 31, 2022