Expression

Target
IDP52003  
Expression ID
A12106  
Protein Clone
pMCSG53 (Oct 27, 2021)  
Experiment Date Start
Oct 31, 2021  
Experiment Date End
Nov 02, 2021  
Person
Christine Tesar  
Lab
Argonne National Laboratory  
Protocol
standard expression_ANL  
Status
success  
Expression Organism
E. coli  
Expression Strain
BL21-Gold(DE3)  
Media Type
LB + 40 mM K2HPO4  
Media Volume
2000 mL  
Growth Temperature
37 °C  
Induction Temperature
18 °C  
Induction Reagent
0.5 mM IPTG  
Experiment Type
production  
Expression Level
80.0 %  
Solubility Level
33.0 %  
Inserted
Jan 31, 2022  

Related Purifications

Experiment
Start
Date		 Experiment
End
Date		 Person Lab Protocol Status Affinity
Tag
Status		 SeMet
Status		 Covalent
Modification		 Final
Buffer		 Shipped
To
Lab		 Shipped
Date