Expression

Target
IDP99024  
Expression ID
A12200  
Protein Clone
pMCSG53 (Nov 22, 2021)  
Experiment Date Start
Apr 26, 2022  
Experiment Date End
Apr 28, 2022  
Person
Natalia Maltseva  
Lab
Argonne National Laboratory  
Protocol
standard expression_ANL  
Status
success  
Expression Organism
E. coli  
Expression Strain
BL21 (DE3) pGrow7-K  
Media Type
LB  
Media Volume
2000 mL  
Growth Temperature
37 °C  
Induction Temperature
16 °C  
Induction Reagent
0.5 mM IPTG  
Experiment Type
production  
Expression Level
70.0 %  
Solubility Level
80.0 %  
Inserted
May 16, 2022  

Related Purifications

Experiment
Start
Date		 Experiment
End
Date		 Person Lab Protocol Status Affinity
Tag
Status		 SeMet
Status		 Covalent
Modification		 Final
Buffer		 Shipped
To
Lab		 Shipped
Date