Expression

Target
IDP00044  
Expression ID
U178  
Protein Clone
pMCSG7 (Apr 21, 2008)  
Experiment Date Start
Aug 24, 2009  
Experiment Date End
Aug 25, 2009  
Person
Majka Klimecka  
Lab
University of Virginia  
Protocol
uva_expression_semet_1  
Status
success  
Expression Organism
E. coli  
Expression Strain
BL21 Codon Plus RILT  
Media Type
SeMet  
Media Volume
4000 mL  
Growth Temperature
37 °C  
Induction Temperature
20 °C  
Induction Reagent
0.5 mM IPTG  
Experiment Type
production  
Solubility Level
80.0 %  
Inserted
Aug 28, 2009  

Related Purifications

Experiment
Start
Date		 Experiment
End
Date		 Person Lab Protocol Status Affinity
Tag
Status		 SeMet
Status		 Covalent
Modification		 Final
Buffer		 Shipped
To
Lab		 Shipped
Date