Expression

Target

IDP90271  

Expression ID

W1  

Protein Clone

pET28A(+) (Oct 02, 2009)  

Experiment Date Start

Oct 15, 2009  

Experiment Date End

Oct 16, 2009  

Person

Chris Nelson  

Lab

Washington University  

Protocol

WU_native_soluble_expression_1  

Status

success  

Expression Organism

E. coli  

Expression Strain

BL21-CodonPlus(DE3)-RIL  

Media Type

LB  

Media Volume

10000 mL  

Growth Temperature

30 °C  

Induction Temperature

25 °C  

Induction Reagent

0.5 mM IPTG  

Experiment Type

production  

Expression Level

10.0 %  

Solubility Level

10.0 %  

Inserted

Oct 21, 2009