Expression

Target
IDP90271  
Expression ID
W3  
Protein Clone
pET28A(+) (Oct 02, 2009)  
Experiment Date Start
Oct 15, 2009  
Experiment Date End
Oct 16, 2009  
Person
Chris Nelson  
Lab
Washington University  
Protocol
WU_native_soluble_expression_1  
Status
success  
Expression Organism
E. coli  
Expression Strain
BL21-CodonPlus(DE3)-RIL  
Media Type
LB  
Media Volume
1000 mL  
Growth Temperature
30 °C  
Induction Temperature
25 °C  
Induction Reagent
0.5 IPTG  
Experiment Type
production  
Expression Level
70.0 %  
Solubility Level
70.0 %  
Inserted
Oct 21, 2009  

Related Purifications

Experiment
Start
Date		 Experiment
End
Date		 Person Lab Protocol Status Affinity
Tag
Status		 SeMet
Status		 Covalent
Modification		 Final
Buffer		 Shipped
To
Lab		 Shipped
Date