Expression

Target

IDP00044  

Expression ID

U218  

Protein Clone

pMCSG7 (Apr 21, 2008)  

Experiment Date Start

Feb 20, 2010  

Experiment Date End

Feb 21, 2010  

Person

Majka Klimecka  

Lab

University of Virginia  

Protocol

uva_expression_semet_1  

Status

success  

Expression Organism

E. coli  

Expression Strain

DE21 COdon Plus RILT  

Media Type

LB  

Media Volume

2000 mL  

Growth Temperature

37 °C  

Induction Temperature

18 °C  

Induction Reagent

0.5 mM IPTG  

Experiment Type

production  

Expression Level

80.0 %  

Solubility Level

80.0 %  

Inserted

Feb 21, 2010