Expression

Target

IDP90289  

Expression ID

N360  

Protein Clone

pET28 (Jun 05, 2009)  

Experiment Date Start

Sep 19, 2009  

Experiment Date End

Sep 20, 2009  

Person

Jeff Jiapeng  

Lab

Northwestern University  

Protocol

NU_SelMet_expression  

Status

success  

Expression Organism

E. coli  

Expression Strain

DE3, KJE, GroEL/S  

Media Type

TB  

Media Volume

1500 mL  

Growth Temperature

37 °C  

Induction Temperature

18 °C  

Induction Reagent

0.4mM IPTG  

Experiment Type

production  

Expression Level

40.0 %  

Solubility Level

40.0 %  

Inserted

Mar 24, 2010