Clone

Target

IDP00157  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

430  

Reference Sequence Used

MRKYQQGVGL LEAILASAVL GMALVAAGSY YKREAELMIK SSNAFDVIEL SSQIQRYASL SKINNRTNPI LKDNKAKEFK DADLKWLKLE NCLTAGDVPT TGNNNDLQDQ FIACDADYRK GDLSYFGSQF EFSTYVHPSN PEIQRQIKQV VSYFQYRGME RAFIGDAAGY VISEAKKKGF SAQDYRIVLI EPDRVGYFES NVISYEEFIE NPSARENFLL KATKDRTLAL AVSLAQTGEI AMQRDGSVAF LEDTELCWDT AAGSAKSCLS VRYDTVGNKT ELDLKQIDVV SAKGLSFESD GKTKTPVVST YETFQDGGRA KTINAIECPT GLNNRFAAVV SSFSTAGQNA NFSSESAKDS QGTTQKDGSK GPHALLSGIS LNWTLTNKVW DVTASIGIES GILPTSGIDS GSLLRNPKSL SFIAFQWCEN  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAGAAAATACCAACAAGGTG  

PCR Backward Primer

TTATCCACTTCCAATGTTAATTTTCACACCATTGAAACG  

Shipped To Lab

ANL  

Shipped Date

Jul 16, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008