Clone

Target

IDP00832  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

560  

Reference Sequence Used

MNQHLLGNPK LTVTHVNEVK AGINHIVVDS VQYGNQEMIM EKDGTVEMRD GEKLYINIFR PNKDGKFPVV MSADTYGKDN KPKITNMGAL WPTLGTIPTS SFTPEESPDP GFWVPNDYVV VKVALRGSDK SKGVLSPWSK REAEDYYEVI EWAANQSWSN GNIGTNGVSY LAVTQWWVAS LNPPHLKAMI PWEGLNDMYR EVAFHGGIPD TGFYRFWTQG IFARWTDNPN IEDLIQAQQE HPLFDDFWKQ RQVPLSQIKT PLLTCASWST QGLHNRGSFE GFKQAASEEK WLYVHGRKEW ESYYARENLE RQKSFFDFYL KEENNDWKDT PHVIYEVRDQ FYKGEFKSAS AFPLPNAEYT PLYLNAENHT LNHAKISSAH VAQYDSEDKQ QDVSFKYTFD KDTELVGNMN LKLWVSTKDS DDMDLFAGIK KLDRRGNEVN FPDFNHIENG QVATGWLRVS HRELDQEKSS IAQPWHKHET ELKLSQDEIV PVEIELLPSG TLFKQGETLE VVVKGSEIVI GNSTPGMKTR YEHEETVNKG MHMIYTGGKY DSQLIIPIVN  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MNQHLLGNPK LTVTHVNEVK AGINHIVVDS VQYGNQEMIM EKDGTVEMRD GEKLYINIFR PNKDGKFPVV MSADTYGKDN KPKITNMGAL WPXLGTIPTS SFNLKDHQTQ DFGCQMIML* LKLHYAVVTN PKASYLHGQK EKRKIITK*L NGQQISHGVM EISGQMVFLI LR*LNGGSHH *IHHI*KQ*F LGKA*MICIE K*PFTEVYQI LAFIVSGLKV FLRDGQIIQI SKI*FKHNKN ILCSMIFGNS VKCHYHKLKH LY*HVLVGLH KVCTTVALLK DLNKLHLKKN GYMCMDVKSG KVTTLEKISN AKNHSLIFTL KKKITIGKIR LMSFMKLEIN FIKANSNQRQ RSLYLTQNIH HCI*MLKITH *IMQRLVARM SHNMTLKINN KM*VLNIRLT KILS*LET*T *NYG*ALKTQ MIWIYLQVLK S*IVVVMKLT SLILIILKMV K*LLVGYAYH IVN*IKKNPQ SRNLGINMKQ N*SCHKMRLY LLKSNCYLQA RYLNKAKHWK LL*RVVKL*L VIVLLA*KHV MNMKKP*IKA CT*FILVVNM IHN*SFLSLI  

PCR Forward Primer

TACTTCCAATCCAATGCGATGAACCAACATTTACTAGGAAA  

PCR Backward Primer

TTATCCACTTCCAATGTCAATTAACGATAGGAATGATTAA  

Shipped To Lab

UT  

Shipped Date

Jun 30, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008