Clone

Target

IDP00016  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

491  

Reference Sequence Used

MKLHTLVSCL HDFPVVPKEN PEITSIEADS RKVKEGSLFV CMKGYTVDSH DFAKQAAAQG AAAIVAERPI DVDVPVVLVK NTFRSLAVLA DYFYGQPTHK LHLIGITGTN GKTTTSHIMD EIMRAHGHKT GLIGTINMKI GDETFEVKNT TPDALTLQQT FAKMVEHGVD STVMEVSSHA LDLGRVHGCD YDVAVFTNLT QDHLDYHKTM EEYKHAKGLL FAQLGNSYNH NREKYAVLNN DDAVTVEYMR STAATVVTYG IDTTSDVMAK DIAMTSGGTT FTLVTPYESV NVTMKLIGKF NVYNVLAATA AGLVSGVSLE TIIDVIKELA GVPGRFEVVD GGQNYTVIVD YAHTPDSLEN VLKTAKQFAK GDVYCIVGCG GDRDRTKRPI MASVATQYAT HAIYTSDNPR SEDPAAILDD MVHGANGNNY EVIIDRKEAI HHAITKAKAD DIIIIAGKGH ETYQIIGKDV HHFDDREVAK EAITERLNNE E  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAGTTGCATACACTTGTATC  

PCR Backward Primer

TTATCCACTTCCAATGTTACTCTTCGTTGTTTAAACGC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008