Clone

Target

IDP00036  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

213  

Reference Sequence Used

MEAFIRSDQY NFIKSQAYIL ANGHATANDR GVIQALKSLA IEKIIHVFEN LTDEQKELID TVLTVQNRED AESFLTKINP YVIPFQEVTA QTLKKLFPKA KKLKLPDMEE MDMKEISYLS WVDKGSSRKF IIAKNDKNKF VGLQGTFQSL NKKSICSLCH GHEEVGMFLV EIKGDIPGTF VKKGNYICKD GVACNQNMKS LDKLQDFIER LKK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGAAGCTTTTATTAGAAGTGA  

PCR Backward Primer

TTATCCACTTCCAATGTTATTTCTTCAATCTCTCAATAAAA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008