Clone

Target

IDP00029  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

360  

Reference Sequence Used

MNNPVVLDSK TELNEQTAQD VRLQLRQDAD VQRIYNAVDI KDQLELIELG KEPSMEISRF ADQILHTMSL SKIEDSGELL KQLGKIMDRF DSKDFAEEKS GFFSRMFKKA DKMIEQIFSK YQTMGREIDK VYVEITKYQD EMKKSIGTLD GLYEQNLKYY LDLEKYVVAG EMLLERLNTE LVPMYEERVR NNDQLAGIEL ESLKNSVEIL EQRIDDLEKA RMVALLTAPQ IRMIQRGNNK LIGKINTAFI TTIPIFKNGI IQAVNAKRQK LVADSMAELD RRTNELLKKN AQNIATQSVE VARLSGSSSI KMETLEETWN IISRGMQETQ QIEEQNKRER EESRKRMATL TENIKKELQG  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAATAACCCAGTCGTACTAG  

PCR Backward Primer

TTATCCACTTCCAATGTTATCCTTGCAATTCTTTTTTTATG  

Shipped To Lab

ANL  

Shipped Date

May 08, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008