Clone

Target

IDP00004  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

446  

Reference Sequence Used

MENISDLWNS ALKELEKKVS KPSYETWLKS TTAHNLKKDV LTITAPNEFA RDWLESHYSE LISETLYDLT GAKLAIRFII PQSQAEEEID LPPAKPNAAQ DDSNHLPQSM LNPKYTFDTF VIGSGNRFAH AASLAVAEAP AKAYNPLFIY GGVGLGKTHL MHAIGHYVIE HNPNAKVVYL SSEKFTNEFI NSIRDNKAVD FRNKYRNVDV LLIDDIQFLA GKEQTQEEFF HTFNALHEES KQIVISSDRP PKEIPTLEDR LRSRFEWGLI TDITPPDLET RIAILRKKAK AEGLDIPNEV MLYIANQIDS NIRELEGALI RVVAYSSLIN KDINADLAAE ALKDIIPNSK PKIISIYDIQ KAVGDVYQVK LEDFKAKKRT KSVAFPRQIA MYLSRELTDS SLPKIGEEFG GRDHTTVIHA HEKISKLLKT DTQLQKQVEE INDILK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGAAAACATCTCTGATTTATG  

PCR Backward Primer

TTATCCACTTCCAATGCTACTTTAAAATATCGTTAATTTCT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008