Clone

Target

IDP00019  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

585  

Reference Sequence Used

MRKTKIVCTI GPASESIEKL EQLIEAGMNV ARLNFSHGSH EEHGARIKNI REASKKTGKT VGILLDTKGP EIRTHDFVDG QAELVTGAEV VLSTEQVLGT AEKFSVSYAG LYDDVDPGSR ILIDDGLIEL EVIEKADGNI RTKVLNSGTV KNKKGVNVPN VSIKLPGITE KDVKDIIFGI EQKVDFIAAS FVRKAADVLE IRELLEEHNA QYIQIVPKIE NQEGIDNIDS ILEVSDGLMV ARGDMGVEIP PEEVPLVQKR LIKKCNVLGK PVITATQMLD SMQRNPRPTR AEASDVANAI FDGTDAIMLS GETAAGQYPV EAVTMMANIA VRVEKSLQYE DMFKKRIKEF TPTITDAISQ SVAHTALALD VAAIVAPTES GYTAKMISKY RPKSPIVAVT SDEQVGRRLA LVWGVQAFMA EKRAASTDEM LDTAIQTGMD AGLIGLGDTV VITAGVPVAE TGTTNLMKIH VVGEEVAKGQ GIGRKAAKGK VVVAKTAAEA VANVNEGDIL VTTSTDKDMI PAIEKAAALV VEEGGLTSHA AVVGVSIGIP VIVGVNGVTA TLKNGQEVTV DAARGIVYNG HAEVL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCGTAAAACTAAAATTGTATGT  

PCR Backward Primer

TTATCCACTTCCAATGTTATAGCACTTCCGCATGTCC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008