Clone

Target

IDP00170  

Clone Suffix

4

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

609  

Reference Sequence Used

MKMIQRPLNW LVLAGAATGF PLYAAQMVTI DDASMVEQAL AQQQYSMMPA ASGFKAVNTV QLPNGKVKVR YQQMYNGVPV YGTVVVATES SKGISQVYGQ MAQQLEADLP TVTPDIESQQ AIALAVSHFG EQHAGESLPV ENESVQLMVR LDDNQQAQLV YLVDFFVASE TPSRPFYFIS AETGEVLDQW DGINHAQATG TGPGGNQKTG RYEYGSNGLP GFTIDKTGTT CTMNNSAVKT VNLNGGTSGS TAFSYACNNS TNYNSVKTVN GAYSPLNDAH FFGKVVFDMY QQWLNTSPLT FQLTMRVHYG NNYENAFWDG RAMTFGDGYT RFYPLVDINV SAHEVSHGFT EQNSGLVYRD MSGGINEAFS DIAGEAAEYF MRGNVDWIVG ADIFKSSGGL RYFDQPSRDG RSIDHASQYY SGIDVHHSSG VFNRAFYLLA NKSGWNVRKG FEVFAVANQL YWTPNSTFDQ GGCGVVKAAQ DLNYNTADVV AAFNTVGVNA SCGTTPPPVG KVLEKGKPIT GLSGSRGGED FYTFTVTNSG SVVVSISGGT GDADLYVKAG SKPTTSSWDC RPYRSGNAEQ CSISAVVGTT YHVMLRGYSN YSGVTLRLD  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAAATGATACAACGTCCTC  

PCR Backward Primer

TTATCCACTTCCAATGTTAGTCCAAGCGTAACGTCAC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008