Clone

Target

IDP00145  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

350  

Reference Sequence Used

MNELMGIRVA SSKRENPTLA GRDYWLMGLM LVALMGLTIS SVFVGSRDIP YAVTFDALFH FDEQNTQHLL VHYLRVPRTG LAILVGAALG AAGVIMQAMT RNPLADPGIL GVNAGAILMV VIGIAGFGLT DMLHYLWFGL FGAAATSVGV YLLAGINQSP NPVRVVLAGA AMTVVLLSMT HLIMLNSAQE VFNQFRHWSI GSLQGRGYEV LLPTSVFVLL GLIAAILLAR GLDTVVLGHD AGKALGVNPL WIWIASAAVV TLLAGTATAA AGPISFLGLT APHLARLWVG AEHRRLLPYS MLLGALLLLG ADLAGRLIGS NDEISAGIMV ALIGGPVFVL LVRRWKLSHL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAATGAGCTGATGGGAATAA  

PCR Backward Primer

TTATCCACTTCCAATGCTACAGATGTGATAGTTTCCAT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008